Mite Removal Report

Mite removal is to eliminate mites. Mites are widely distributed in the dark corners of the living room, carpets, mattresses, pillows, sofas, air conditioners, and coolers, among which mattresses are the most suitable environment for mites to grow and reproduce. Bedding in the home should be cleaned frequently. Mites are tiny animals, usually about 0.5 mm in size, with many hairs on their bodies and feet, and mouthparts at the front end, which are dander-loving and generally have a life span of 3 months, but have a strong reproductive capacity, and one mite can become 300 in 3 months.

Mite removal test report

Vacuum cleaner mite removal test? Mite removal testing laboratory? -Shenzhen Fangliu testing, a professional mite removal testing organization, provides you with one-stop service.

For UV vacuum cleaners, UV lamps, ozone disinfection machines and other products, many foreign buyers began to require the production of such products began to require sterilization, mite testing, to test the performance of the product to meet the relevant requirements. At present, the test can cover more than 20 species, such as Escherichiacoli, Salmonella, P. aeruginosa, Staphylococcus and so on.

Detection of anti-mold materials and products include: fabrics and textile products, synthetic polymeric materials, wood, cardboard and paper products, leather, packaging materials, plastics and plastic products, biodegradable materials, paints, lacquers, paint films and adhesives, electronic and electrical products, military products, fungicidal anti-mold agents, washed feathers and down, synthetic latex, new nano anti-bacterial anti-mold materials, photocatalytic anti-bacterial anti-mold materials and other materials.

Antimicrobial materials and products testing items:

Detection of antibacterial materials and products include: fabrics and textile products, packaging materials, plastics and plastic products, ceramics, paints, lacquers, lacquer and adhesives, fungicides, washed feathers and down, synthetic latex, new nano antibacterial materials, photocatalytic antibacterial and anti-mold materials, etc.

Testing items: antibacterial performance and antibacterial effect, evaluation of behavior under the action of microorganisms, microbial resistance, antibacterial performance, etc.

Test equipment and materials

1. Stereo microscope (LEITZ)

2. Constant temperature and humidity incubator

3. Petri dishes (58mm glass petri dishes)

4. Covered vessel (200mm glass Petri dish with special cover, 50 mm ventilation holes, covered with PTFE film)

5. Sticky plate (square glass plate with side length of 180mm)

6. Powdered feed for mites (provided by Shenzhen University, composition compound GB/24253-2009 Appendix A example of nutrient composition)

7. Mite counting tools: counter, dissecting needle, brush

8. Saturated salt water

9. Balance, ** to 0.01g

Specimen preparation

1. Cut the fabric into a 58mm circle, 3 specimens, 3 control samples (provided by your organization)

2. Place the specimens under the dry heat condition of 65℃ for 10min

3. Washing of specimens: 3.3.1 Detergent: ECE (B) detergent, we buy finished products. 3.3.2 Washing conditions: refer to GB/T12490-2007 for A1M washing. one cycle of A1M is equivalent to 5 washes, refer to AA standard, 20 washes are needed, i.e. 4 A1M cycles. (A cycle for: 150mL solution to add ** 10 grains, washing at 40 ℃ for 45min, remove the specimen in 100mL 40 ℃ water swelling wash twice, each time 1min). 4 cycles after full washing with water, air dry.

Detection methods:

1. Household dust mite collection and screening

Household dust mites were collected from the natural environment, and according to the mite's photophobic habit, adult mites were moved by the short irradiation method with fluorescent tubes, and adult mites were collected by separating them with brushes under an optical microscope.

2. Irradiation mite killing test

Twenty-five adult mites were placed in a 5.0 cm diameter and 2.0 cm high glassware, and the handheld UV sterilizer was placed directly above the large glassware (2.0 cm from the mites) for irradiation. The irradiation method was performed according to the product instructions. The irradiation dose and grouping were based on the results of the pre-test, i.e., three irradiation dose groups were set: 4.83 min, 7.0 min and 9.0 min at the appropriate distance from the handheld UV sterilizer, and the activity of dust mites was observed under the optical microscope immediately after irradiation, and the survival of dust mites was observed at 1h, 12h and 24h after irradiation, and inactivation was determined by final inactivity. was determined. The kill rate was calculated [kill rate = (number of test dust mites - number of surviving dust mites)/number of test dust mites. The test sites were repeated four times.

A control group was set up at the same time as the mite killing test, with the same environmental conditions except no UV irradiation was added. The survival of dust mites was observed at different times.

Test method and procedure

1. Place a 10 mm thick sponge with a side length of 200 mm in the bottom of the covered container.

2. Fill with saturated salt water. The height of the sponge should be just submerged  

3. Take 7 petri dishes, a petri dish placed in the center of the sticky plate as the central petri dish, the remaining 6 around the center into a petal-shaped evenly placed, and the edge between each petri dish with the same width of transparent tape sticky, and then the 7 petri dishes fixed on the sticky plate.  

4. Place (2000±200) surviving mites on the central Petri dish.

5. In **6 petri dishes, put the test and control samples respectively at intervals, lay the test samples evenly, flatly and closely on the bottom of the petri dishes, and put 0.05g of mite feed in the center of the test samples.

6. Place the sticky board assemblies with test mites and feed on the sponge, cover the top lid of the container, and incubate in a constant temperature and humidity incubator (temperature 25℃, relative humidity 75%) 4.7 After 24h incubation, observe with a stereomicroscope and use appropriate methods to calculate the number of surviving adult mites and worm mites in the petri dishes of the specimen and the petri dishes of the control sample.